Open-book assignment 2Part 1ProjectYour company is growing plants that produce a very valuable...

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Open-book assignment 2Part 1ProjectYour company is growing plants that produce a very valuable chemical X in their floral petals. Genes responsible for the biosynthesis of this metabolite are Gene XA, GeneXB and GeneXC. These genes express only in petals because of the petal-specific transcription factor, TFX, which regulates them.These plants cannot be used for the commercial production of X because:they do not produce many flowers;their flowers are small in size andflowering is seasonal since these plants flower only in spring and flowering lasts just a few weeks.As a result, it is not easy to collect enough biomass from petals every year for the commercial production of X.To increase the production of the chemical X your company decided to re-direct the production of chemical X from flowers to leaves of the same plants. The rationales behind this decision are:these plants have big leaves,leaves’ growth is not seasonal’ andleaves and flowers have the same genome, and all genes involved in X production exist in leaves.You are the lucky postdoc who has to run this project. You need to design a strategy to conduct this project. To commercialize the generated transgenic plants under field conditions, they have to meet modern (advanced) genetic engineering conditions.You need to:Design a transgene and explain its every component (you can design more than one transgene): 20 marksDesign a vector that contains your transgene: 20 marksDesign a strategy for the generation of the transgenic plant: 20 marksDesign a strategy to confirm your generated transgenic plant: 10 marksExpression of genes X in leaves could change the expression profiles of other genes. How can you assess the global molecular changes in leaf cells? : 20 marksWhich adverse effects do you expect in your experiment: 10 marksYour report:A 1000  word review. You can add pictures and diagrams if required. Use paragraphs to separate different parts of the review. Show in bold critical components of the gene silencing mechanisms.You must clearly address a research topic ? The information presented must be accurate and logically organised ? You should demonstrate an innovative solution to an industry challenge/problem.

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Genes X may alter the expression patterns of other genes when they are expressed in leaves. How can the molecular alterations in leaf cells be evaluated on a global scale?
A free-growing aspen (Populus tremula) was used to study gene expression in its leaves over the course of multiple growing seasons. The temperature influenced the quantity of chlorophyll that accumulated and how rapidly this programme progressed. The research might be utilised to identify genes that serve as biomarkers for certain phases or therapies, as well as to determine how spontaneous variations in gene expression occur. Leaves are formed from the apical meristems of a plant as it grows. "Determinate growth," on the other hand, is a characteristic of certain trees. Because all of next year's leaves have been created, they will remain in buds until the following spring.
You can learn a lot about how the environment and development interact by studying plants in controlled or uncontrolled situations. Using data from aspen trees growing in their natural context, we were able to distinguish environmental and genetic influences. Aspen trees have three phases of life for their leaves. It has been shown that gene expression is more variable during the start and end of the season, as determined by measurements of the expression of eleven different genes. When leaves begin to develop, chlorophyll begins to build up within a week. This process continues for another two weeks after the leaves have stopped growing (Figures 1 and 2).
Temperature is thought to be the most critical component in leaf development from the time the bud opens until the leaf is completely developed. Genes in the eleven selected dates were studied using DNA microarrays. Temperatures ranged from 10°C to 20°C throughout the day on the selected days. When the weather wasn't as typical as prior days, we also utilised a sample taken on July 18. (rainy). Before July 1, most of the changes in gene expression that occur throughout development take place. This is the time of year when the leaves are developing and forming their interior structures.
In 2002, it was exceptionally warm at this time, while in May and June of 2000, it was abnormally chilly. Gene expression in leaf development may be influenced by temperature.

Open-book assignment 2Part 1 Project Your company is growing plants that produce a very valuable chemical X in their floral petals. Genes responsible for the biosynthesis of this metabolite are Gene...

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