(Given a 30L of (liquid) bioreactor installed with 3 six-blade turbine (flat-blade) impeller...

Question

(Given a 30L of (liquid) bioreactor installed with 3 six-blade turbine (flat-blade)
impeller (W/D=1/5), the diameter of impeller is 10 cm, diameter of tank DT = 50 cm ,
impeller rotation speed N = 150 rpm, air aeration rate Fa = 30 L
min
, the density of the
culture media (ρL) is 1g cm3 ⁄ , dynamic viscosity of the culture media μL = 10−2
g
cm ∙ sec
.
E. coli with a qO2
of 10 mmol O2⁄(g cells ∙ h) is to be cultured; oxygen solubility in the
liquid CL = 2 mg O2/L. Saturated dissolved oxygen concentration (C*) in the liquid at 1atm
and 25°C is 7 mg/L.
a) What maximum concentration of E. coli can be sustained in this bioreactor under
aerobic conditions?
b) What concentration of cells could be maintained if pure oxygen was used to spare the
reactor?

Dr Shweta · Accepted Answer

Solution
Given: 
30L of liquid bioreactor with 3 six flat--blade turbine, W/D of impeller =1/5, impeller diameter =10 cm, tank diameter DT = 50 cm, rotation speed of impeller N = 150 rpm, air aeration rate Fa = 30 L/min, culture media density (ρL) =1g/cm3, dynamic viscosity of media μL = 10−2g/cm ∙ sec, qO2 of E. coli = 10 mmol O2⁄ (g cells ∙ h), solubility of oxygen in liquid CL = 2 mg O2/L.

(Given a 30L of (liquid) bioreactor installed with 3 six-blade turbine (flat-blade) impeller (W/D=1/5), the diameter of impeller is 10 cm, diameter of tank DT = 50 cm , impeller rotation speed N = 150...

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